May 1, 2018

Hugo Silva

10.00-10.15  Hugo Silva
Life and Health Sciences Research Institute (ICVS), Braga, Portugal

A glycolytic lactate-based metabolism underlies chicken lung branching morphogenesis

Lung branching morphogenesis is an intricate process governed by mesenchymal-epithelial interactions and dependent on a complex signaling. To sustain high proliferative rates, free energy and building blocks are necessary. The metabolic requirements of the embryonic lung are unknown, thus we characterized the metabolic profile of early chick lung branching. Ex vivo explant culture, 1H-NMR, qPCR, in situ hybridization and western blot were performed. Results revealed lactate and acetate as developmental biomarkers, and branching morphogenesis showed a glycolytic lactate-dependent metabolism.

 

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