10.15-10.30 Marta Shahbazi
University of Cambridge, UK
“Defining pluripotency in 3D: from embryonic stem cell identity to morphogenesis”
Pluripotency refers to the capability of a cell to generate all the cell types of the organism. Different pluripotent states are identified in vivo and in vitro, but their biological significance with respect to morphogenesis remains unclear.We show that exit from an unrestricted naïve state directs epithelialization in ESCs and embryos. This process is controlled by Oct4, which mediates expression of sialomucins, required for lumen opening. Naïve cells polarize but fail to open a lumen. Likewise, mouse and human embryos locked in the naïve state fail to form the amniotic cavity.