14.15-14.30 Rebecca Lea
The Francis Crick Institute, London, UK
“Identifying novel regulators of human pluripotency and embryogenesis”
The mode of specifying pluripotent human epiblast (hEPI)is unclear and may differ from the mouse. Recent advances in single-cell techniques have allowed investigation of gene and transcriptional networks in human pre-implantation development. Numerous transcription factors (TFs), including KLF17, VENTX and ARGFX, are enriched in hEPI, upregulated in “naïve” hESCs, but absent in mouse blastocysts. I am investigating the role of theseTFs in pluripotency in hESCsthrough both loss- and gain-of-function studies, aiming to define human-specific pluripotency regulators acting in vitro and in vivo.