12.15-12.30 Tom Watson
The Francis Crick Institute, London, UK
“The Gene Regulatory Network Underlying the Neural-to-Glial Fate Transition in the Developing Spinal Cord”
Neurons and glia are generated sequentially in the central nervous system. In vivo, this process is under tight spatial and temporal control. Although several factors have been defined, the underlying gene regulatory network mediating the transition remains unclear. Using single-cell transcriptomics, we define the transcriptional changes that accompany the transition and identify novel candidate genes involved. With CRISPR-Cas9 and lentiviral technologies, we test these candidates and reveal a novel player in the activation of the glial lineage.